Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae)

Nidularium minutum is an ornamental bromeliad from the Brazilian Rainforest. The micropropagation of this species essential for obtaining plants available conservation programs or commercial use. Our study aimed to establish efficient plant production method by in vitro sprouting. This takes a long time sprout vitro, and 10% produce shoots culture medium without growth regulators (PGRs). When subcultured PGR-free medium, these individualized can like mother plant. Murashige Skoog basal (MS) containing 1.0 mg L-1 6-benzylaminopurine (BAP) promoted induction adventitious greater than 90% after 240 days with average more eight per Approximately 100% vitro-produced survived acclimatization, reaching flowering stage. Therefore, our results showed that regeneration N. depends on cultivation period higher sprouting capacity be selected used as matrices, contributing endangered bromeliad.